1. An experiment was conducted to test the effects of a plasma calcium activating agent in a sample of pigeons of both sexes. The results of the experiment are shown below. Determine whether the drug had an effect on plasma calcium in general, whether the sex of the bird makes a difference and any interaction between the two variables.
   No Hormone Treatment Hormone Treatment

   Female	Male	Female	Male
   16.5	14.5	39.1	32.0
   18.4	11.0	26.2	23.8
   12.7	10.8	21.3	28.8
   14.0	14.3	35.8	25.0
   12.8	10.0	40.2	29.3

2. To access the impact of industrial waste on aquatic life, the respiratory rate (ml O ₂ /hr) of three species of fiddler crabs (both sexes) was determined. Other data collected were the water temperature and the species of crab found at each site. The data follows.
   Species I
   Low temperature Med temperature High temperature

   Male	Female	Male	Female	Male	Female
   1.9	1.8	2.3	2.4	2.9	3.0
   1.8	1.7	2.1	2.7	2.8	3.1
   1.6	1.4	2	2.4	3.4	3.0
   1.4	1.5	2.6	2.6	3.2	2.7

   
   Species I I
   Low temperature Med temperature High temperature

   Male	Female	Male	Female	Male	Female
   2.1	2.3	2.4	2.0	3.6	3.1
   2.0	2.0	2.6	2.3	3.1	3.0
   1.8	1.9	2.7	2.1	3.4	2.8
   2.2	1.7	2.3	2.4	3.2	3.2

   
   Species II I
   Low temperature Med temperature High temperature

   Male	Female	Male	Female	Male	Female
   1.1	1.4	2.0	2.4	2.9	3.2
   1.2	1.0	2.1	2.6	2.8	2.9
   1.0	1.3	1.9	2.3	3.0	2.8
   1.4	1.2	2.2	2.2	3.1	2.9

   
   Determine whether there is a significant difference in respiratory rates among species, whether temperature plays any role and whether the sex of the organism is important. Look at all of the interactions among the three factors.
3. The dataset below shows the velocity of an enzyme catalyzed reaction which follows Michaelis Menton kinetics. Enter the data into SigmaPlot and produce two journal-ready figures: Michaelis Menton and Lineweaver-Burke. Both figures are to be on the same page. Include a text box showing the Vmax and Km values.
   DATA:

   Substrate concentration(mM)	0.2	0.3	0.4	0.5	1
   Velocity (µM/Min)	105.3	142.9	166.7	181.8	256.4

4. (A) Analyze the file ozone.sav ( File is attached ) to determine the best equation that describes the data. The output file should contain the figure with the data and your fit selection, the exact equation and the reason(s) for this selection.

(B) Import the ozone.sav file ( File is attached- same ) into SigmaPlot and perform a similar curve fitting study. Compare the results you obtained with your conclusions from the SPSS analysis. Import the SigmaPlot figure into a word document along with your conclusions.

Price: $19.3

Solution: The downloadable solution consists of 12 pages, 730 words and 17 charts.
Deliverable: Word Document